#sdspage

MOST RECENT

Protein pertamakali diidentifikasi oleh umat manusia pada tahun 1838. Semenjak itu, bentuk, ragam, dan fungsinya selalu menjadi topik menarik hingga saat ini.
Mengidentifikasi protein merupakan hal yang sangat menarik, kita mungkin belum pernah melihat secara fisik per molekulnya, namun manfaatnya sudah terasa, ya kita hidup karena protein.
Mengidentifikasi protein kata expert akan 'lebih sulit' dari gen. Misal, setelah kita berbangga hati dapat mengisolasi protein dari suatu sampel berdasarkan berat molekulnya (Mr) dengan SDS PAGE 1 dimensi, ternyata dalam satu band terdapat beberapa jenis protein (ya karena cuma berdasarkan Mr). Ada metode lebih presisi dengan memisahkan berat molekul dan titik isoelektrik yaknk 2D SDS PAGE. Lebih dari itu, ternyata ada metode lain yang lebih lebih presisi dan cepat serta memanfaatkan ion misal MALDI-TOF atau LC-MS/MS, (yang jumlah alatnya di Indo mungkin tidak lebih dari jari tangan kanan kita yang ga ngapa2in pas ngetik chat).
Bahkan kombinasi antara SDS PAGE - LC-MS/MS - Bioinformatika dapat menjadi metode yang kuat dan cepat dalam mengidentifikasi suatu protein!
Ilmu pengetahuan dan teknologi terus berkembang, menjadi kelemahan atau kelebihan, tantangan atau ancaman, tergantung kita meletakkannya.
*ps band pada SDS PAGEnya ndlemok karena pas pelatihan ga diukur kadar protein sampelnya, senggaknya enggak smiling :) .
#protein #sdspage #unsoed

Despite the bad smell of B mercapto ethanol, it is my favorite solvent... Need for lineer protein . Protein boiled for westernblot...
#westernblot #experimentalscience #laboratory #proteinexpression #solvents #merck #chemistry #biochemistry #medicalbiology #scienceteacher #biotechnology #funnyexperiment #sigmaaldrich #sdspage

Mondays means Westerns 😏.
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Fingers and toes crossed, I have been doing this experiment for almost two years now 🙃, and provided these blots are nice (I am for once pretty confident as I have already ran these samples before), that will be the experiment all wrapped up and figures + sup. figures all done for my paper!! 😁.
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(p.s I know it's Tuesday but for some reason my insta wasn't letting me post anything yesterday 🤔😣).
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#science #laboratory #labwork #lablife #scientist #scientificresearch #scienceblogger #womeninstem #womeninscience #femalescientist #phd #phdlife #researchstudent #cancerresearch #phdstudent #girlsinscience #womanwhoscience #STEMsquad  #wearescience #westenblot #westernblotting #gelelectrophoresis #proteinanalysis #proteinextraction #sdspage

Ini adalah trik untuk memilih pewarna atau staining dalam analisis protein dengan teknik SDS PAGE dan WB.. pemilihan staining umumnya dipengaruhi dari jumlah protein yg diperoleh saat ekstraksi.. untuk mengetahui spesifikasinya, silakan diamati tabel berikut ini.. no 1-3 untuk SDS PAGE, no 4 WB.. 👍
Selamat belajar.. 🤗🤗 #westernblotting #sdspage #geldeproteina #ponceuas #nitratodeprata

As proteínas que são transferidas do gel SDS-PAGE para a membrana podem ser marcadas ainda quando estão no gel mas também quando já estão na membrana de nitrocelulose ou de PVDF. Para a coloração direta do gel podemos usar Comassie-Blue ou por tratamentos com nitrato de prata.
O nitrato de prata é um dos métodos mais sensíveis de detecção.
Para a coloração das proteínas na membrana utilizamos o Ponceau S.
Observe as marcações na foto. #westernblotting #sdspage #geldeproteina #ponceuas #nitratodeprata

I remember seeing this gel for the first time after taking it out of the destaining solution just like yesterday.
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Months after trying to prove the encapsulation of my hybrid artificial enzyme into a protein cage, multiple failed experiments and near-breakdown moments, I saw what I needed to see. I felt pure happiness, as if I had just discovered a cure for cancer 😅
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Doing a PhD is hard, we all know that. Just remember that there's always hope at the end of the tunnel, guys. If you feel lost, anxious and down, talk to someone and try to keep going, positive results are simply waiting for you to find them ♥️
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#sdspage #protein #enzyme #encapsulation #proteinengineering #catalysis #biochemistry #biology #molecularbiology #biotechnology #sci #motivation #phd #phdlife #electrophoresis #proteingel #hanginthere #youcandoit #semester #gradschool

separace proteinů z bakterií, které exprimují 3 enzymy ze ze čmeláka skalního, pomocí gelové elektroforézy

separation of proteins from bacteria expressing 3 enzymes from red-tailed bumblebee using gel electrophoresis

#electrophoresis #sdspage #bacteria #biochemistry #chemistry #biochemist #protein #enzyme #uochb #science #veda #czech

3 Layanan Utama INBIO
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Analisis #Bioinformatika

Analisis Hasil #Sequencing (#sanger dan #NGS)
Analisis potensi #herbal
Analisis #MolecularDocking
Analisis Simulasi (#VirtualCell)
Analisis Pathway (#GeneratePathway)
Analisis #Proteomics (Peptide sequencing MASCOT, PPI)
Analisis #Genomics (#Mutation , #phylogenetics and stc.)
Analisis #Transcriptomics (Expressions data, GEO, R studio)
Analisis baru sesuai dengan permasalahan penelitian .
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Analisa Molekuler

#DNASequencing
#IsolasiDNA dan PCR
#Blotting (Western Blot)
#SDSPAGE
#ELISA
#FlowCytometry
#Chromatography analysis (HPLC. LCMS, GCMS)
Peptide Sequencing
#Histology (HE, IHK, IHF)
#CellCulture
#AnimalModelCare
#Biostatistika .
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Workshop

Inbio juga mengadakan workshop peningkatan skill bioinformatika (jadwal tertera di web)

PRIVATE TRAINING juga tersedia apabila ada permintaan topik-topik tertentu. Waktu menyesuikan dengan permintaan peserta

#inbioindonesia #bioinformatics #biologimolekuler #bioinformatika #workshop

Follow @inbio_indonesia
Visit us at http://www.inbio-indonesia.org

When your supervisor is away on holiday it means you get to chill out a bit, right?
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Wrong (at least in my case😂). My supervisor has an unrealistic expectation when it comes to experiments and work load 😂. When he goes away he gives me 10x more work than humanly possible. So to meet this expectation, today I ran a total of EIGHT western blots alongside a little PCR inbetween 😂🙃. This makes 16 blots this week so far 😊.
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I think I am going a little crazy..
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#science #laboratory #labwork #lablife #scientist #scientificresearch #scienceblogger #womeninstem #womeninscience #femalescientist #phd #phdlife #researchstudent #cancerresearch #phdstudent #girlsinscience #womanwhoscience #STEMsquad  #wearescience #westernblotting #westernblot #blottingfordays #gelelectrophoresis #sdspage

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